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KMID : 1007520020110030257
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Food Science and Biotechnology
2002 Volume.11 No. 3 p.257 ~ p.259
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Microbiological Identification of Beans Exposed to Gamma Irradiation (DEFT/APC Method)
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Oh, Kyeung Nam
Lee, Sook Young/Yang, Jae Seung
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Abstract
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A microbiological method (DEFT/APC) based on direct epifluorescent technique (DEFT) and the conventional total aerobic plate count (APC) was used for the detection of irradiation treatment of beans Soybeans, kidney beans and red beans were used for the screening test of gamma irradiation. These samples were irradiated at below 1.0 kGy (0.1, 0.3, 0.5, 0.7, 1.0) and identified using DEFT/APC method. The log DEFT/APC units increased gradually in all samples with irradiation because the DEFT counts (total microorganisms) were nearly changed but the APC counts decreased with the dose increment. Samples irradiated with a dose level of at least 0.5 kGy showed 2.0 or higher in logarithmic unit. These results indicate the DEFT/APC method could be used for screening irradiation treatment of beans.
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